5-ethynyl-2'-deoxycytidine  (Jena Bioscience)

Journal: Aging Cell

Article Title: Plasma Dilution After Myocardial Ischemia–Reperfusion Injury Promotes Cardiac Repair, Heart Performance, and Recovery of Motor Function and Endurance in Old Mice

doi: 10.1111/acel.70525

Figure Lengend Snippet: NBE restores de novo cardiac proteome levels to normal. (A) Schematic diagram. NBE was performed 24 h post‐injury. Mice were injected with azido‐homo‐alanine for 5 days starting with the onset of injury. Heart samples were harvested at 5 days post injury. (B) Principal component analysis (PCA) plots of the protein expression profile of all treatment groups showed clear separation of sham and I/R controls, with clustering of I/R + NBE group in sham. Confidence ellipses at 50%, 80%, and 95% intervals are overlaid to indicate group variance. (C) Volcano plots identified differentially expressed proteins (DEPs) in I/R versus Sham controls and I/R + NBE versus I/R controls. Proteins highlighted in red are significantly upregulated while proteins highlighted in blue are significantly downregulated (adjusted p ‐value < 0.05) (horizontal dotted line), and log2 fold change > 2 (vertical dotted line). (D) Venn diagram showed the 12 overlapping DEPs between the upregulated proteins after injury and their downregulation after NBE treatment. (E) Heatmap visualized the expression of the 12 overlapping proteins across all groups. (F, G) Gene Ontology overrepresentation analysis of the 12 overlapping DEPs highlighted top 10 biological processes and molecular function, respectively. (H) cirFunMap visualization of KEGG enriched pathways of the 12 overlapping proteins.

Article Snippet: After injury, mice were injected intraperitoneally with azido‐homo‐alanine (Jena Biosciences CLK‐AA009, 0.02 mmol/kg) daily for 5 days.

Techniques: Injection, Expressing

Journal: bioRxiv

Article Title: Chlamydia trachomatis deploys sphingolipids for genome organisation

doi: 10.64898/2026.04.29.721357

Figure Lengend Snippet: HeLa 229 WT (a), HeLa WT (e, f) or HeLa CERT K.O.(e, f) cells were infected with C. trachomatis in presence (f) or absence (a-e) of TFSM2 for the indicated duration. Then either RNA was extracted and expression of host cell SMases (SMPD1-SMPD4) were measured by RT-qPCR (a, b), cells were lysed and cellular neutral sphingomyelinase (NSM, c) as well as acid sphingomyelinase (ASM, d) activity was determined or samples were fixed, immunostained for chlamydial HSP60, CERT and DNA (e). Scale bar: 50 µm. Samples incubated with TFSM2 (e), were stained with BODIPY-FL-DBCO and AZDye546-azide, 4-fold expanded and FRET efficiencies in inclusions and host cell areas were determined. FRET efficiency ratios “inclusion vs. host cell” were calculated. n≥3. Statistics: Two-way RM ANOVA and Šidák’s multiple comparison.

Article Snippet: Subsequently, alkyne groups of TFSM derivatives were stained with AZDye546 azide [Jena Bioscience, Cat. No. CLK-1283; product discontinued and we recommend using AZDye546-picolyl-azide (Jena Bioscience, Cat. No. CLK-1284) alternatively] for 1 h/37°C at 170 rpm.

Techniques: Infection, Expressing, Quantitative RT-PCR, Activity Assay, Incubation, Staining, Comparison